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浙江大学学报(工学版)
材料科学与化学工程     
掺杂及分子修饰对类金刚石细胞相容性的影响
刘敏1 ,刘爱萍1,2 ,郁建灿1,钱国栋1
1.浙江大学 硅材料国家重点实验室,材料科学与工程学院,浙江 杭州310027;
2.浙江理工大学 物理系,浙江 杭州 310018
Influence of doping and molecular modification on cell compatibility of diamond like carbon film
LIU Min1, LIU Ai ping1,2, YU Jian can1, QIAN Guo dong1
1. State Key Laboratory of Silicon Materials, School of Material Science and Engineering, Zhejiang University, Hangzhou 310027, China; 2. Department of Physics, Zhejiang Sci Tech University, Hangzhou 310018, China
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摘要:

为实现类金刚石(DLC)薄膜在不同使用环境下生物相容性的可调性,采用元素掺杂及化学分子修饰对DLC薄膜表面进行改性,借助X射线光电子能谱、拉曼光谱及静态接触角测试表征薄膜的表面形貌和微观结构,探究掺杂及分子修饰对薄膜细胞相容性的影响.结果显示:B、N、P、Pt元素掺杂以及3 氨基苯硼酸(APBA)修饰均没有改变薄膜非晶结构,只是提高薄膜有序程度.B、N、P元素的掺入减小了DLC薄膜的接触角,而N、Pt元素共掺使接触角变化不大.APBA修饰使各种DLC薄膜的接触角均减小.B、N、P元素的掺入促进PC12细胞的黏附与增殖,抑制细胞凋亡,而Pt元素的掺入起相反的作用,APBA修饰对细胞生长有明显促进作用.这说明元素掺杂及分子修饰是调节DLC薄膜细胞相容性的有效方法.

Abstract:

DLC film was functionalized by element doping and molecular modification aiming at the biocompatibility regulation of diamond like carbon (DLC) film for better use in different environment as the substrate material of cell sensor. X ray photoelectron spectroscopy, Raman spectroscopy and static state contact angle measurement were applied to characterize the surface morphology and microstructure of DLC film. The effects of element doping and molecular modification on the cell compatibility of the film were explored. Results show that the doping of B, N, P and Pt elements and the modification of 3 aminobenzeneboronic acid (APBA) do not change the amorphous structure of DLC film, but enhance the ordered structure. The doping of B, N and P elements decreases the contact angle of DLC film, while the codoping of N and Pt elements does not lead to obvious change of  contact angle. The APBA modification makes the contact angle decrease for different DLC films. Moreover, the doping of B, N and P elements promotes the adhesion and proliferation of PC12 cells and inhibits cell apoptosis. However, the incorporation of Pt element presents the opposite effect. The modification of 3 aminophenylboronic acid can obviously promote the cell growth on the doped DLC surface. Therefore, element doping and molecular modification are effective methods for biocompatibility modulation of DLC film.

出版日期: 2015-10-15
:  TB 321  
基金资助:

国家自然科学基金资助项目(51272237,51272231,51010002);中国博士后科学基金特别资助项目(2013T60587);中国博士后科学基金资助项目(2012M520063);浙江省博士后基金资助项目(Bsh1201016).

通讯作者: 钱国栋, 男, 教授. ORCID: 0000 0001 7133 2473.     E-mail: gdqian@zju.edu.cn
作者简介: 刘敏(1989-), 女,硕士生, 从事薄膜生物相容性研究. ORCID: 0000 0002 5032 4973. E-mail: aipingwz@163.com
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引用本文:

刘敏,刘爱萍,郁建灿,钱国栋. 掺杂及分子修饰对类金刚石细胞相容性的影响[J]. 浙江大学学报(工学版), 10.3785/j.issn.1008 973X.2015.09.024.

LIU Min, LIU Ai ping, YU Jian can, QIAN Guo dong. Influence of doping and molecular modification on cell compatibility of diamond like carbon film. JOURNAL OF ZHEJIANG UNIVERSITY (ENGINEERING SCIENCE), 10.3785/j.issn.1008 973X.2015.09.024.

链接本文:

http://www.zjujournals.com/eng/CN/10.3785/j.issn.1008 973X.2015.09.024        http://www.zjujournals.com/eng/CN/Y2015/V49/I9/1790

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